Thermo Fisher Scientific APBA1 Polyclonal Antibody
Thermo Fisher Scientific의 APBA1 Polyclonal Antibody는 Human, Mouse, Rat 시료에 반응하며 WB, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. Rabbit IgG 폴리클로날 항체로, Protein A로 정제되었으며 1 mg/mL 농도의 액상 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human APBA1 (amino acids 451–550) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The munc-18 interacting protein (Mint) family consists of evolutionarily conserved adaptor proteins involved in membrane transport and organization. In mammals, three isoforms exist: Mint1, Mint2, and Mint3. While the N-terminal regions vary, the C-terminal regions are highly conserved and contain phosphotyrosine-binding (PTB) and PSD-95/DLG-A/ZO-1 (PDZ) domains that mediate protein interactions.
Mint1 and Mint2 are primarily expressed in the brain and interact with Munc18, a key component of synaptic vesicle fusion. Mint3, expressed ubiquitously but at lower levels in the brain and testis, does not interact with Munc18. Instead, Mint3 binds to the Alzheimer’s disease-related amyloid precursor protein (APP) through its PTB and PDZ domains, potentially linking APP to transport machinery and regulating its endocytosis and metabolism. Abnormal APP metabolism is associated with early-onset Alzheimer’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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