Thermo Fisher Scientific DNA Ligase III Recombinant Rabbit Monoclonal Antibody (5I7T5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5I7T5

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human DNA Ligase III/LIG3 (NP_039269.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DNA Ligase III,uniProtId:P49916-1,ncbiNodeId:9606,antigenRange:400-500,antigenLength:1009,antigenImageFileName:MA5-51407_DNA_Ligase_III_P49916-1_Rabbit.svg,antigenImageFileNamePDP:MA5-51407_DNA_Ligase_III_P49916-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.09 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_3093807

Product Specific Information

Immunogen sequence: IASRCTANDL KCIIRLIKHD LKMNSGAKHV LDALDPNAYE AFKASRNLQD VVERVLHNAQ EVEKEPGQRR ALSVQASLMT PVQPMLAEAC KSVEYAMKKC P

Target Information

There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100 kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97 kDa (beta form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.