Thermo Fisher Scientific RUNX1T1 Polyclonal Antibody
RUNX1T1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체입니다. Western blot, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합하며, 고순도의 항원 친화 크로마토그래피로 정제되었습니다. 동결 건조 형태로 제공되며, 재구성 후 500 µg/mL 농도로 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 0.5–1 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC-F) | 0.5–1 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | - |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived human RUNX1T1/ETO recombinant protein (Position: T335–D510) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2747058 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia (particularly the M2 subtype), the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities.
This translocation produces a chimeric gene composed of the 5′ region of the RUNX1 gene fused to the 3′ region of the RUNX1T1 gene.
The resulting chimeric protein is believed to associate with the nuclear corepressor/histone deacetylase complex, thereby blocking hematopoietic differentiation.
Several transcript variants encoding multiple isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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