Thermo Fisher Scientific PRMT5 Polyclonal Antibody
PRMT5 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, IP 등에 사용 가능. FFPE 조직에서 citrate buffer(pH 6.0) 전처리 권장. 4°C 보관, 액상 형태로 제공되며 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 580 and the C-terminus (residue 637) of Protein Arginine Methyltransferase 5 (PRMT5) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with X. laevis, bovine, and orangutan
Target Information
PRMT5 (Protein Arginine N-Methyltransferase 5) is an arginine methyltransferase that catalyzes the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for MMA.
PRMT5 mediates the symmetrical dimethylation of arginine residues in small nuclear ribonucleoproteins Sm D1 and Sm D3, a process essential for snRNP core particle assembly and biogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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