Thermo Fisher Scientific CD223 Monoclonal Antibody (333210)
Human LAG-3(CD223)에 특이적인 Mouse monoclonal antibody. Western blot에 최적화되어 있으며, 교차 반응 없음. 단백질 A/G로 정제된 동결건조 형태로 제공. PBS와 trehalose buffer에 재구성하여 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 333210 |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human LAG-3 (Leu23–Leu450) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609285 |
Product Specific Information
- In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse LAG-3 is observed.
- Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
LAG-3 is a 70-kDa surface glycoprotein belonging to the immunoglobulin superfamily with homology to CD4.
It binds to MHC class II with higher affinity than CD4 and is involved in the negative regulation of T cell activation and homeostatic proliferation.
Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells.
CD8+ T cells generally express higher levels of LAG-3 than CD4+ T cells.
Coexpression of LAG-3 and CD49b has been proposed as a marker for Type 1 regulatory T cells (Tr1 cells) in both humans and mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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