Thermo Fisher Scientific SARS-CoV-2 S1 Protein Ig Total Human ProcartaPlex Simplex Kit

Invitrogen™ SARS-CoV-2 S1 Protein Ig Total Human ProcartaPlex™ Simplex Kit

The Human SARS-CoV-2 S1 Protein Ig Total Simplex ProcartaPlex Kit measures anti-SARS-CoV-2 S1 protein antibodies in serum and plasma. The detector antibody is an Immunoglobulin (Ig) Total antibody directly conjugated to phycoerythrin (PE) that cross-reacts with all Ig (IgG, IgM, and IgA) in serum and plasma. IgG is the most abundant of the immunoglobulins.

This kit is designed to be combinable with other Simplex kits from the Coronavirus Panel so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection and quantitation. When combining multiple Simplex kits, only one Viral Reagent kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex assays for the Luminex platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 targets with the MAGPIX instrument.
ProcartaPlex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.

주요 특징

• Flexible panels — Design your own panels with Simplex kits to measure your own array of targets
• More results per sample — Measure up to 80 protein targets in a single 25–50 μL sample
• Well-established Luminex technology — The most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems).
Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. Each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE).

The main difference from a traditional ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead rather than adsorbed to a microplate well, allowing reagents to remain free-floating in solution. The Luminex 200 instrument uses two lasers — one to distinguish bead signatures and another to quantify RPE fluorescence, which is proportional to the amount of protein present.

ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel. More than 90% of ProcartaPlex Simplex targets can be combined, providing superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine).
For more information, visit thermofisher.com/procartaplex.

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