Thermo Fisher Scientific Alpha-1-Antitrypsin (SERPINA1) Recombinant Rabbit Monoclonal Antibody (AAT, 3167R)
Recombinant rabbit monoclonal antibody targeting Alpha-1-Antitrypsin (SERPINA1). Validated for WB and IHC applications. High specificity and reproducibility with HEK293 expression system. Supplied as liquid, 200 µg/mL, for research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | AAT, 3167R |
| Immunogen | Recombinant human Alpha-1-Antitrypsin (AAT) protein fragment (amino acids 41–179) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Alpha-1-Antitrypsin (Alpha-1-AT) is synthesized in the liver and acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin.
Deficiency leads to chronic obstructive lung disease (emphysema) and can cause hepatic cirrhosis in infants.
Alpha-1-AT levels increase during acute phase responses to tissue necrosis and inflammation, and are elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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