Thermo Fisher Scientific TRAF5 Polyclonal Antibody
TRAF5 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot 및 파라핀 포매 조직 면역조직화학(IHC) 분석에 적합. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol 완충액에 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fusion protein corresponding to a region derived from internal residues of human TNF receptor-associated factor 5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2635731 |
Product Specific Information
The antibody detects endogenous levels of total TRAF5 protein.
Target Information
Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g., RANK, CD30, CD40, etc.) and the interleukin-1 receptor.
The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Molecular cloning studies have identified six TRAFs (TRAF1–TRAF6). TRAF5 is a 558-amino acid protein implicated in NF-κB and c-Jun N-terminal kinase/stress-activated protein kinase activation by members of the TNF receptor superfamily, including CD27, CD30, CD40, and lymphotoxin-beta receptor.
Targeted disruption of the TRAF5 gene causes defects in CD40-CD27 mediated lymphocyte activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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