Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), PerCP-Cyanine5.5, eBioscience

Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), PerCP-Cyanine5.5, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.03 µg/test
  View 49 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 737 / 805
• Brilliant Violet 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 610-30S
• NovaFluor Red 685 / 710
• NovaFluor Yellow 570 / 590 / 660
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA).
This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage cells.
CD24 is variably glycosylated, resulting in heterogeneity of molecular mass across different lineages.
Antibodies to CD24 show subtle differences in staining levels among lymphocyte populations.
CD24 expression helps resolve stages of B lymphopoiesis in mouse bone marrow.
It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes; ≤ 0.03 µg/test recommended.
A test is defined as the amount of antibody staining a cell sample in 100 µL final volume.
Cell number can range from 10⁵–10⁸ cells/test.
Careful titration is recommended for optimal performance.

Excitation: 488 nm
Emission: 695 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD24 is a two-chain GPI-anchored glycoprotein expressed at multiple stages of B-cell development, starting from bone marrow pro-B cells to mature surface Ig-positive B cells.
Plasma cell expression is low or absent.
CD24 is expressed in most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas.
It may regulate B-cell proliferation and maturation and contribute to autoimmunity control.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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