
Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), PerCP-Cyanine5.5, eBioscience
마우스 CD24 항원(HSA)에 특이적인 M1/69 단클론 항체로, PerCP-Cyanine5.5 형광표지되어 있음. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 0.03 µg/test로 사용 권장. B세포 발달 단계 분석 및 CD24 관련 연구용으로 적합.
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Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), PerCP-Cyanine5.5, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.03 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-Cyanine5.5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/69 |
| Conjugate | PerCP-Cyanine5.5 |
| Excitation / Emission Max | 489 / 679 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1210702 |
Additional Formats
- Unconjugated
- Alexa Fluor 532
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 737 / 805
- Brilliant Violet 711 / 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 510 / 610-30S
- NovaFluor Red 685 / 710
- NovaFluor Yellow 570 / 590 / 660
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645
- Request custom conjugation
Product Specific Information
The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA).
This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage cells.
CD24 is variably glycosylated, resulting in heterogeneity of molecular mass across different lineages.
Antibodies to CD24 show subtle differences in staining levels among lymphocyte populations.
CD24 expression helps resolve stages of B lymphopoiesis in mouse bone marrow.
It has been reported that P-selectin (CD62P) binds to CD24.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes; ≤ 0.03 µg/test recommended.
A test is defined as the amount of antibody staining a cell sample in 100 µL final volume.
Cell number can range from 10⁵–10⁸ cells/test.
Careful titration is recommended for optimal performance.
Excitation: 488 nm
Emission: 695 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD24 is a two-chain GPI-anchored glycoprotein expressed at multiple stages of B-cell development, starting from bone marrow pro-B cells to mature surface Ig-positive B cells.
Plasma cell expression is low or absent.
CD24 is expressed in most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas.
It may regulate B-cell proliferation and maturation and contribute to autoimmunity control.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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