Thermo Fisher Scientific Histone H2A.X VHH-8His-Cys-tag Recombinant Alpaca Monoclonal Antibody (SAA1353)

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: Assay-dependent

ELISA

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Compatible with Direct ELISA

Target Information

Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome core particle. In eukaryotes, DNA double-strand breaks (DSBs) trigger phosphorylation of serine 139 at the carboxy terminus of H2AX, resulting in gamma-H2AX. This phosphorylation can be detected by Western blotting or immunofluorescence to reveal DSB frequency.

Phosphatidylinositol 3-kinases are implicated in H2AX phosphorylation, though it remains unclear whether ATM is the primary kinase or if DNA-PK and ATR also contribute. Structurally, H2A.X contains 143 amino acids and is synthesized in both G1 and S phases. Its mRNA includes polyA addition motifs and a polyA tail, along with conserved stem-loop and U7 binding sequences involved in histone mRNA stability.

Two forms of H2A.X mRNA exist: one ~1600 bases long with polyA, and another ~575 bases without polyA. The short form behaves as a replication-type histone mRNA, while the long form acts as a basal-type histone mRNA. Histone H2A.X maps to the 11q23.2–q23.3 region of the human chromosome and contributes to histone formation and DNA structural integrity. The H2A variant H2A.X specifically regulates the interaction of MDC1, a DNA repair protein, at sites of DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.