Thermo Fisher Scientific CD45RA Monoclonal Antibody (HI100), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD45RA Monoclonal Antibody (HI100), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test

---

Product Specifications

---

Additional Formats

• Unconjugated
• Alexa Fluor 532, 700, Plus 555, Plus 750
• APC, APC-eFluor 780
• Biotin, Brilliant UV/Violet series
• eFluor 450, 506
• FITC
• NovaFluor Blue 800, Red 710, UV 765, Violet 690, Violet 800, Yellow 610, Yellow 660, Yellow 810
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702
• Custom conjugation available

---

Product Specific Information

The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ and CD8+ peripheral T lymphocytes and B cells. CD45RA+ T cell populations are mainly naive/virgin, allowing HI100 mAb to serve as a phenotypic marker for T cell subset discrimination.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells at 5 µL (0.8 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Note:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):
Required for optimal staining with NovaFluor conjugates. Add 5 µL per sample, regardless of the number of NovaFluors used. For single-color controls, use 5 µL per 100 µL of cell sample containing 10³–10⁸ cells.

NovaFluor conjugates are based on Phiton™ technology utilizing nucleic acid dye structures for engineered fluorescent signatures, minimizing spillover and spread impacts.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

---

Target Information

CD45RA is an isoform of the CD45 complex expressed in specific lymphoid subtypes. CD45 (leukocyte common antigen) is a receptor-type protein tyrosine phosphatase found in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases, lymphocyte development, and antigen signaling. Isoforms differ by extracellular domain splicing and influence membrane localization and signaling behavior.

CD45 plays roles in:

• Immunoreceptor signaling regulation
• Integrin-mediated survival signaling
• DNA fragmentation during apoptosis

Expressed on hematopoietic lineage cells except mature red cells. Dysfunction is linked to severe combined immunodeficiency and Hepatitis C.

---

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

---

제품 이미지