Thermo Fisher Scientific HPV Type 16 E7 Monoclonal Antibody (8C9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:2

-

Immunocytochemistry (ICC/IF)

-

View 6 publications 6 publications

Immunoprecipitation (IP)

1:10

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8C9

Immunogen

Bacterially derived fusion protein containing the human papillomavirus tp 16 early protein E7 open reading frame.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

50 µg/mL

Purification

HPLC

Storage buffer

PBS with 1% BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533057

Product Specific Information

In Western blot against the HPV16-E7 bacterially derived fusion protein, the antibody detects a 52 kD band which is composed of the HPV16-E7 protein (15 kDa) and the trpE protein (37 kDa). Positive reactivity for the 15 kDa HPV16-E7 expressed protein was shown with CaSki (HPV16 DNA-containing cell line) cells by Western blot. The antibody has also been shown to immunoprecipitate and immunocytochemically stain the HPV16-E7 expressed protein from the CaSki cell line. Negative reactivity by Western blot was shown with a series of other HPV fusion proteins including: HPV16-E2, HPV16-E4, HPV16-E6, HPV18-E7, HPV6-E7, HPV6-E4, and HPV6-L1. The antibody failed to react with HeLa (HPV18 DNA-containing cell line) cells and other HPV DNA negative cervical carcinoma cell lines, HT-3 and C-33A, by Western blot, by immunoprecipitation or by immunocytochemical staining analyses.

Reconstitute the lyophilized material with 1 mL of deionized or distilled water for a final concentration of 50 µg/mL.

Target Information

The HPV E7 proteins are small (HPV16 E7 comprising 98 amino acids, 15kDa), zinc binding phosphoproteins which are localised in the nucleus. They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses. The first 16 amino-terminal amino acids of HPV16 E7 contain a region homologous to a segment of the conserved region 1 (CR1) of the E1A protein of subgenus C adenoviruses. The next domain, up to amino acid 37, is homologous to the entire region 2 (CR2) of E1A. Genetic studies have established that these domains are required for cell transformation in vitro, suggesting similarities in the mechanism of transformation by these viruses. The CR2 homology region contains the LXCXE motif (residues 22-26) involved in binding to the tumour suppressor protein pRb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.