Thermo Fisher Scientific p19Arf Monoclonal Antibody (12.A1.1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1:5,000-1:20,000

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Hamster, Human, Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

12.A1.1

Immunogen

This protein G purified monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 54-75 of mouse p19Arf protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p19ARF,uniProtId:Q64364-1,ncbiNodeId:10090,antigenRange:54-75,antigenLength:169,antigenImageFileName:200-501-892_p19ARF_Q64364-1_Rat.svg,antigenImageFileNamePDP:200-501-892_p19ARF_Q64364-1_Rat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Directed against mouse p19Arf protein. No reactivity is observed against human or hamster homologues. The epitope was putatively mapped to amino acids 54-62 of mouse p19Arf. BLAST analysis indicates that no significant sequence homology exists for this sequence with p19Arf homologues from other sources. This epitope is also found on mouse p16Ink4a protein. No additional information is available for reactivity with p19Arf protein from other sources.

Target Information

In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.