Thermo Fisher Scientific Phospho-Bora (Ser497) Polyclonal Antibody

Applications

• Western Blot (WB): 1:1,000–1:3,000

Product Specifications

Target Information

Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly, and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2-dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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