Thermo Fisher Scientific Phospho-BCLAF1 (Ser531) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human BCLAF1(Accession Q9NYF8), corresponding to amino acid residues around phosphorylated Ser531. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BCLAF1,uniProtId:Q9NYF8-1,ncbiNodeId:9606,antigenRange:531,antigenLength:920,antigenImageFileName:PA5-118678_BCLAF1_Q9NYF8-1_Rabbit.svg,antigenImageFileNamePDP:PA5-118678_BCLAF1_Q9NYF8-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2903178

Product Specific Information

Antibody detects endogenous levels of BCLAF1 only when phosphorylated at Ser531.

Target Information

BCLAF1 is an intracellular, death promoting transcriptional repressor of survival genes that interacts with several members of the BCL2 family of proteins. It shows homology to the basic leucine zipper (bzip) and MYB DNA binding domains.From recent research it can be concluded that in case of apoptotic cells this protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope. When over expressed this protein induces apoptosis and can be suppressed by co-expression of BCL2 proteins. Also known as BTF, it triggers a proapoptotic signal, potentially helpful to overcome the resistance to STI571.BCLAF1 is found to express mostly in skeletal muscles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.