Thermo Fisher Scientific Nephrin Monoclonal Antibody (174CT2.1.1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

-

Immunocytochemistry (ICC/IF)

1:10-1:50

-

Product Specifications

Host/Isotype

Mouse / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

174CT2.1.1

Immunogen

KLH conjugated synthetic peptide between 1088-1117 amino acids from human Nephrin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Nephrin,uniProtId:O60500-1,ncbiNodeId:9606,antigenRange:1088-1117,antigenLength:1241,antigenImageFileName:MA5-37488_Nephrin_O60500-1_House_mouse.svg,antigenImageFileNamePDP:MA5-37488_Nephrin_O60500-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Lot-Specific

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2897421

Target Information

Primary steroid resistant nephrotic syndrome (SRNS) is characterized by childhood onset of proteinuria and progression to end stage renal disease. Kidney podocytes and their slit diaphragms form the final barrier to urinary protein loss. Congenital nephrotic syndrome (CNS) is caused by mutations in NPHS1 (nephrin) or NPHS2. Nephrin, a recently identified protein is a member of a group of podocyte proteins that constitute major component of the slit diaphragm especially in the foot process. Nephrin, a cell adhesion molecule, may play a crucial role in maintaining the glomerular filtration barrier. Recent studies have suggested that mutations in the gene for Nephrin reportedly lead to congenital nephrosis. Three novel podocyte proteins, Podocin, Nephrin and alpha Actinin 4 have been identified in congenital and experimental models of proteinuria. The role of Nephrin in anti apoptotic activity in podocyte slit diaphragm is believed to be associated with vascular endothelial derived growth factors VEGF signaling. Nephrin seems to play a role in the development or function of the kidney glomerular filtration barrier and it may anchor the podocyte slit diaphragm to the actin cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.