Thermo Fisher Scientific EGFP/GFP/Venus Polyclonal Antibody
EGFP, GFP, Venus 단백질을 인식하는 sheep polyclonal antibody로, WB 및 IHC에 적합. 합성 펩타이드 면역원 사용. 비결합형, 동결건조 형태로 제공되며 장기 보관 시 -20°C 권장. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | – |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | – |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptides from EGFP/GFP/Venus protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity: EGFP/GFP/Venus.
Target Information
The jellyfish Aequorea victoria contains the green fluorescent protein (GFP Tag) that emits light in its bioluminescence reaction. GFP is widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP enables localization studies, movement tracking, and analysis of subcellular compartment dynamics. GFP technology has provided new insights into living cell physiology.
GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically. It absorbs blue light (max 395 nm) and emits green light (peak 508 nm) without additional proteins or cofactors. GFP fluorescence is stable, species-independent, and applicable to numerous research purposes, including protein localization, interaction studies (yeast two-hybrid), and FRET experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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