Thermo Fisher Scientific CDK9 Polyclonal Antibody
Rabbit polyclonal antibody targeting CDK9, validated for WB, IHC, ICC/IF, and ELISA. Recognizes CDK9 residues 350–C-terminus. Predicted to react with mouse, rat, and cow. Suitable for research on cell cycle regulation and transcriptional control.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:250 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| ELISA | 0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human CDK9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10983208 |
Product Specific Information
This antibody is predicted to react with mouse, rat, and cow based on sequence homology.
Target Information
CDK9 is a serine/threonine kinase that forms an active holoenzyme complex with Cyclin T1, playing a key role in regulating the eukaryotic cell cycle. It is coexpressed and copurified with Cyclin T1 and regulates cytokine-inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity toward CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and may predispose to heart failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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