Thermo Fisher Scientific MRPL21 Polyclonal Antibody
Human MRPL21 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS와 40% glycerol buffer에 보관. 연구용으로 적합하며, 미토콘드리아 리보솜 단백질 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human MRPL21. Recombinant protein control fragment (Product #RP-100632). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2644132 |
Product Specific Information
Immunogen sequence:
ACSHSILRPS GPGAASLWSA SRRFNSQSTS YLPGYVPKTS LSSPPWPEVV LPDPVEETRH HAEVV
Antigen sequence identity:
- Mouse: 83%
- Rat: 82%
Target Information
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Unlike prokaryotic ribosomes, mammalian mitoribosomes lack a 5S rRNA. The proteins comprising the mitoribosome differ greatly in sequence and biochemical properties among species, making recognition by sequence homology difficult. This gene encodes a 39S subunit protein, and multiple transcript variants encoding different isoforms have been identified, though some may be subject to nonsense-mediated decay (NMD).
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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