Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 5 µL (0.8 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 737, 805
• Brilliant Violet 421, 605, 650, 711
• eFluor 450, 506
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Red 710, 725
• NovaFluor Yellow 590, 660
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

Description:
The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed by developing and mature B cells but not plasma cells. Low expression may occur on a subset of mature T cells. CD20 is implicated in B-cell activation.

Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported:
The 2H7 antibody is reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and validated for flow cytometric analysis of normal human peripheral blood cells.
Use at 5 µL (0.8 µg) per 100 µL test volume. Cell number range: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability detection.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).
• NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures optimized for minimal spillover and spectral spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and most malignant B cells, as well as some T lymphocytes and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation.
CD20 remains on the cell surface after receptor ligation and is a key therapeutic target for B-cell depletion in malignancies.
Mutations or dysfunctions in CD20 are associated with Ms4a1-related common variable immune deficiency.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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