Thermo Fisher Scientific Phospho-LCK (Tyr394) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

A suggested positive control for Western blot is JK cells.

Target Information

LCK is a member of the Src-family tyrosine kinases, essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates ITAM motifs in zeta subunits, creating binding sites for the SH2 domains of ZAP70, which is subsequently phosphorylated and activated by LCK. This activation leads to phosphorylation of adaptor LAT and downstream signaling.
Most LCK localizes to the plasma membrane, with a fraction associated with the Golgi apparatus, potentially contributing to Raf activation under weak TCR stimulation. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2/SH3 domains for protein-protein interactions.
LCK also regulates apoptosis induced by various stimuli (excluding death receptor pathways). Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively spliced variants of the LCK gene encode different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.