Thermo Fisher Scientific EpCAM Recombinant Rabbit Monoclonal Antibody (6H12)
EpCAM 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot과 파라핀 조직 면역조직화학에 적합합니다. 고순도 친화 크로마토그래피 정제, 인간 시료 반응성, 액상 형태로 제공되며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 6H12 |
| Immunogen | A synthesized peptide derived from human EpCAM (target family: EpCAM (CD326), UniProt ID: P16422-1, antigen range: 81–100, antigen length: 314) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.58 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 150 mM NaCl |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2898268 |
Product Specific Information
This antibody has been tested in direct-ELISA.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in epithelial tissues with a molecular weight of approximately 40 kDa. It functions as a calcium-independent epithelial cell adhesion molecule and influences cell cycle, proliferation, and metabolism of epithelial cells and fibroblasts by inducing c-myc and cyclins A and E. Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is associated with enhanced epithelial proliferation and is a known marker for tumors of epithelial lineage. Ep-CAM is highly expressed in human carcinomas, located on the basolateral surface of most simple epithelia, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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