Thermo Fisher Scientific NGF Recombinant Rabbit Monoclonal Antibody (5F10)
Recombinant rabbit monoclonal antibody targeting NGF, validated for WB and IHC(P). High specificity and affinity, produced in HEK293 cells. Supplied as liquid form (3 mg/mL) with PBS/glycerol buffer. Suitable for research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5F10 |
| Immunogen | A synthesized peptide derived from human NGF (aa 212–225) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 3 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 150 mM NaCl |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2898262 |
Product Specific Information
This antibody has been tested in direct-ELISA.
Target Information
Nerve growth factor (NGF) influences the survival and differentiation of various neuronal and nonneuronal cells. Its biological activity results from binding to specific cell surface receptors, present as high and low affinity sites. Only the high affinity form mediates signal transduction. Cross-linking studies with ¹²⁵I-NGF show that the high affinity receptor of rat PC12 cells forms a 158 kDa complex with NGF, while the low affinity receptor forms a 100 kDa complex. Human and rat NGF receptor genes exhibit over 90% homology. NGF stimulates growth and differentiation of sympathetic and sensory neurons and is involved in their regulation. Mutations in the NGF gene are linked to hereditary sensory and autonomic neuropathy type 5 (HSAN5), and dysregulation of NGF expression is associated with allergic rhinitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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