Thermo Fisher Scientific PADI4 Monoclonal Antibody (OTI5B7), TrueMAB
Human PADI4 단백질(aa 299-588)을 항원으로 한 Mouse IgG2a 단클론 항체. Western blot 및 Flow cytometry에 사용 가능. Lyophilized 형태로 제공되며 PBS buffer(8% trehalose) 포함. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Flow Cytometry (Flow)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5B7 |
| Immunogen | Human recombinant protein fragment (aa 299–588 of human PADI4, produced in E. coli) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Catalyzes the citrullination/deimination of arginine residues in proteins.
Citrullinates histone H3 at Arg-8 and/or Arg-17 and histone H4 at Arg-3, preventing methylation by CARM1 and HRMT1L2/PRMT1 and repressing transcription.
Citrullinates EP300/P300 at Arg-2142, enhancing its interaction with NCOA2/GRIP1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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