Thermo Fisher Scientific CEP170 Polyclonal Antibody
CEP170 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ICC/IF, IP, ChIP-Seq 등에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 4°C에서 보관합니다. 세포골격 및 미세소관 조직 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
| ChIP-sequencing (ChIP-Seq) | 10 µg/IP |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1534 and the C-terminus (residue 1584) of human centrosomal protein 170kDa (CEP170, UniProt ID: Q5SW79-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For ICC, formaldehyde fixation is recommended.
- Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Target Information
The CEP170 protein is a component of the centrosome, the major microtubule-organizing center in animal cells. During interphase, it localizes to the sub-distal appendages of mature centrioles, aiding in centrosome organization. During mitosis, it associates with spindle microtubules near centrosomes. CEP170 interacts with and is phosphorylated by polo-like kinase 1, contributing to microtubule organization and cell morphology. The human genome contains a putative pseudogene, and several alternatively spliced transcript variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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