Thermo Fisher Scientific CD304 (Neuropilin-1) Monoclonal Antibody (TNKUSOHA), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.06 µg)/test

Product Specifications

Additional Formats

• Alexa Fluor 700
• APC-eFluor 780
• eFluor 450
• PE-eFluor 610
• Request custom conjugation

Product Specific Information

The TNKUSOHA monoclonal antibody recognizes human CD304 (Neuropilin-1, BDCA-4). CD304 is expressed on human plasmacytoid dendritic cells in blood and bone marrow, T follicular helper cells in inflamed tonsils, and some B-cell acute lymphoblastic leukemias. It is also found in non-hematopoietic cells such as neurons, endothelial, and tumor cells.

Plasmacytoid dendritic cells play a key role in innate immune responses against viral pathogens and tumors.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested for human peripheral blood cells at 5 µL (0.06 µg) per test
• Recommended Cell Number: 10⁵–10⁸ cells/test
• Excitation/Emission: 488 nm / 710 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

PerCP-eFluor® 710 emits at 710 nm and is excited with a 488 nm blue laser. It can replace PerCP-Cyanine5.5. Recommended filter: 710/50 bandpass (695/40 acceptable). Ensure instrument compatibility.

Light Sensitivity: Protect vial and stained samples from light due to photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency.

Target Information

Neuropilin 1 is a 923-amino acid membrane-bound coreceptor belonging to the neuropilin family. It contains two CUB domains, two F5/8 type C domains, and one MAM domain. Neuropilin 1 forms heterodimers with Neuropilin 2 (NRP2) and binds plexin (PLXNB1) to form semaphorin receptors.

It is mainly expressed in the nervous system and is involved in:

• Cardiovascular development and angiogenesis
• Axon guidance and neuronal circuit formation
• Cell survival and migration
• Organogenesis outside the nervous system

Neuropilin 1 functions as a receptor for semaphorins and VEGF, regulating VEGF-induced angiogenesis. It may be associated with advanced prostate carcinoma and breast cancer progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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