Thermo Fisher Scientific GRP94 Polyclonal Antibody
GRP94 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, Flow Cytometry에 적합. Protein A 및 항원 친화 크로마토그래피로 정제. PBS buffer, 0.09% sodium azide 함유, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Hamster, Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 16–43 amino acids from the N-terminal region of human HSP90B1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2542324 |
Product Specific Information
This antibody is predicted to react with bovine, chicken, porcine, non-human primate, and rat based on sequence homology.
Target Information
GRP94 is an 803 amino acid protein belonging to the heat shock protein 90 family. It functions as a molecular chaperone involved in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulate CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC).
It plays a central role in innate and acquired immunity, dendritic cell maturation and chemotaxis, antibody production, and cross-priming. GRP94 serves as a potential marker in breast cancer and acts as a peptide acceptor in the endoplasmic reticulum, aiding peptide loading onto MHC class I molecules.
Expression of GRP94 suppresses A23187-induced apoptosis and stabilizes calcium homeostasis. GRP94 is expressed in melanoma, liver metastases of colon carcinoma cells, and human gastric carcinoma BGC-823 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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