Thermo Fisher Scientific TDRKH Monoclonal Antibody (PCRP-TDRKH-1H2)
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TDRKH 단백질을 인식하는 Mouse IgG2b 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 인간 시료 반응성, 단백질 A/G 정제, 4°C 보관. piRNA 생합성 경로 관련 연구에 적합.
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Thermo Fisher Scientific TDRKH Monoclonal Antibody (PCRP-TDRKH-1H2)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Immunoprecipitation (IP) | 1–2 µg/100–500 µg lysate |
| Peptide Array (Array) | Assay-dependent |
| Immunoelectrophoresis (IE) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCRP-TDRKH-1H2 |
| Immunogen | Recombinant full-length human TDRKH protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is stable for 24 months.
- Positive Control: HeLa or MCF7 cells, human testis or brain.
- Cellular Localization: Cytoplasm; localized to cytosol and centrosome.
- Specificity Comments: Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and maintain germline integrity. The piRNA metabolic process mediates repression of transposable elements during meiosis through complexes of piRNAs and Piwi proteins, governing methylation and transposon repression. Required for the final steps of primary piRNA biogenesis by processing 31–37 nt intermediates into mature piRNAs. May function in pi-bodies and piP-bodies transferring piRNA precursors between granules.
- Predicted to React With: Mouse and Rat.
Target Information
TDRKH is a protein of unknown function containing one tudor domain and two KH domains. It interacts with PIWIL1 and PIWIL4 by binding to methylated arginine residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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