Thermo Fisher Scientific TDRKH Monoclonal Antibody (PCRP-TDRKH-1H2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

1-2 µg/mL

-

Flow Cytometry (Flow)

1-2 µg/10^6 cells

-

Immunoprecipitation (IP)

1-2 µg/100-500 µg lysate

-

Peptide Array (Array)

Assay-dependent

-

Immunoelectrophoresis (IE)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

PCRP-TDRKH-1H2

Immunogen

Recombinant full-length human TDRKH protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TDRKH,uniProtId:Q9Y2W6-2,ncbiNodeId:9606,antigenRange:1-561,antigenLength:561,antigenImageFileName:11022-MSM1-P1_TDRKH_Q9Y2W6-2_House_mouse.svg,antigenImageFileNamePDP:11022-MSM1-P1_TDRKH_Q9Y2W6-2_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Antibody is stable for 24 months.

Positive Control: HeLa or MCF7 cells.Human testis or brain. Cellular Localization: Cytoplasm. Localized to cytosol and centrosome.

Specificity Comments: Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.

Predicted to react with Mouse and Rat

Target Information

TDRKH is a protein of unknown function containing one tudor domain, and two KH domains. It has been found to interact with PIWIL1 and PIWIL4 by binding to methylated arginines residues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.