Thermo Fisher Scientific CD22 Monoclonal Antibody (eBio4KB128 (4KB128)), FITC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Available Formats

• Unconjugated
• APC
• NovaFluor Blue 690
• NovaFluor Red 755
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2), a member of the siglec subgroup of the Ig superfamily.
CD22 is a type I transmembrane glycoprotein composed of two polypeptide chains (CD22α and CD22β, 130–140 kDa) produced by alternative splicing.
It is highly expressed on mature B cells and B cell lymphomas. The extracellular region contains seven Ig-like domains capable of binding sialic acid ligands, while the intracellular region includes six tyrosine residues within ITIM and activation-like motifs that regulate B-cell receptor signaling.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells.
Use 5 µL (0.25 µg) per test in 100 µL final volume. Suitable for 10⁵–10⁸ cells/test.
Excitation: 488 nm Emission: 520 nm Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD22 (BL-CAM) is a type I integral membrane glycoprotein (130–140 kDa) expressed in both cytoplasm and membrane of B-lymphocytes.
It appears early in B-cell differentiation (similar to CD19), with membrane expression limited to mature B cells (CD22⁺) before plasma cell stage (CD22⁻).
CD22 is strongly expressed in hairy cell leukemia, diffuse large B-cell lymphoma, and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
It preferentially binds α2,6-linked sialic acid, and upon ligand-induced phosphorylation, regulates B-cell receptor signaling via interactions with Src family kinases and SH2-domain phosphatases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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