Thermo Fisher Scientific VPS35 Monoclonal Antibody (8A3), APC
VPS35 단백질을 인식하는 마우스 모노클로날 항체로, APC 형광 표지되어 있습니다. Western blot, IHC, ICC/IF, IP에 사용 가능하며, 사람, 마우스, 랫트에 반응합니다. 단백질 G 정제, 1 mg/mL 농도, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8A3 |
| Immunogen | Full length recombinant human VPS35 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932396 |
Additional Formats
- FITC (Cat. No. MA5-45943)
- PerCP (Cat. No. MA5-45944)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45942 was sufficient for detection of VPS35 in 10 µg of SH-SY5Y lysate by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody. Detects approximately 92 kDa.
Target Information
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of the retromer complex, which mediates retrograde transport of proteins from endosomes to the trans-Golgi network. Structural similarity between yeast and human retromer proteins suggests conserved function. Expression studies indicate direct interaction with VPS35, serving as the core of the retromer complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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