Thermo Fisher Scientific VPS35 Monoclonal Antibody (8A3), FITC
VPS35 단백질을 검출하기 위한 FITC 결합 단클론 항체. Western blot, IHC, ICC/IF, IP 등 다양한 응용 가능. Human, Mouse, Rat 반응성. 단클론(Mouse IgG1) 형식으로 고순도 Protein G 정제. 4°C 암소 보관.
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Thermo Fisher Scientific VPS35 Monoclonal Antibody (8A3), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8A3 |
| Immunogen | Full length recombinant human VPS35 |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932397 |
Additional Formats
- APC (Cat. MA5-45942)
- PerCP (Cat. MA5-45944)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45943 was sufficient for detection of VPS35 in 10 µg of SH-SY5Y by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Detects approximately 92 kDa.
Target Information
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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