Thermo Fisher Scientific Topoisomerase II alpha Monoclonal Antibody (TOP2A, 1362)
Topoisomerase II alpha 단백질을 인식하는 Mouse monoclonal 항체. Western blot, IHC, ICC/IF 등 다양한 응용 가능. Human 시료 반응성. Protein A/G 정제, 1 mg/mL 농도의 액상 형태. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TOP2A, 1362 |
| Immunogen | Recombinant fragment of human Topoisomerase II alpha (around aa1352–1493) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues, heat sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes before staining.
Target Information
Topo II alpha plays essential roles in DNA synthesis, transcription, and chromosomal segregation during mitosis.
It serves as a sensitive and specific marker of late S-, G2-, and M-phases in transformed and developmentally regulated normal cells.
Topo II alpha is also implicated in tumor cell drug resistance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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