Thermo Fisher Scientific RAD18 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 216-495 of human Rad18 (NP_0645503) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAD18,uniProtId:Q9NS91-1,ncbiNodeId:9606,antigenRange:216-495,antigenLength:495,antigenImageFileName:PA5-89233_RAD18_Q9NS91-1_Rabbit.svg,antigenImageFileNamePDP:PA5-89233_RAD18_Q9NS91-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.48 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805435

Product Specific Information

Immunogen sequence: INKHLDSCLS REEKKESLRS SVHKRKPLPK TVYNLLSDRD LKKKLKEHGL SIQGNKQQLI KRHQEFVHMY NAQCDALHPK SAAEIVREIE NIEKTRMRLE ASKLNESVMV FTKDQTEKEI DEIHSKYRKK HKSEFQLLVD QARKGYKKIA GMSQKTVTIT KEDESTEKLS SVCMGQEDNM TSVTNHFSQS KLDSPEELEP DREEDSSSCI DIQEVLSSSE SDSCNSSSSD IIRDLLEEEE AWEASHKNDL QDTEISPRQN RRTRAAESAE IEPRNKRNRN; Positive Samples: K-562, MCF-7, Jurkat, 293T, Mouse liver; Cellular Location: Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center

Target Information

Human Rad18 (hRad18) is homologue of the S. pombe Rad18 (3,4). In yeast, Rad6 and Rad18 play a crucial role in post-replication repair functions in gap-filling of a daughter strand on replication of damaged DNA (2). Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring-finger motif (3,4). It has been suggested that Rad18-Rad6 complex binds to the damaged sites by the single-stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by ubiquitin-conjugating enzyme activity of Rad6 (1). The human Rad18 maps on chromosome 3p24-25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56 kDa. The human Rad18 is ubiquitously expressed in various human tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.