Thermo Fisher Scientific ECD/ SGT1 Monoclonal Antibody (PCRP-ECD-1D10)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1-2 µg/mL

-

Flow Cytometry (Flow)

1-2 µg/10^6 cells

-

Immunoprecipitation (IP)

1-2 µg

-

Peptide Array (Array)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

PCRP-ECD-1D10

Immunogen

Recombinant full-length human ECD protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ECD,uniProtId:O95905-1,ncbiNodeId:9606,antigenRange:1-644,antigenLength:644,antigenImageFileName:11319-MSM1-P1ABX_ECD_O95905-1_House_mouse.svg,antigenImageFileNamePDP:11319-MSM1-P1ABX_ECD_O95905-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Predicted to react with Rabbit, Goat, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Orangutan, Elephant (based on sequence homology)

Target Information

Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270). May play a role in regulation of pre-mRNA splicing (PubMed:24722212). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.