Thermo Fisher Scientific NCAPH2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:2,500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human NCAPH2. Recombinant protein control fragment (Product #RP-103867). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NCAPH2,uniProtId:Q6IBW4-1,ncbiNodeId:9606,antigenRange:113-200,antigenLength:605,antigenImageFileName:PA5-64393_NCAPH2_Q6IBW4-1_Rabbit.svg,antigenImageFileNamePDP:PA5-64393_NCAPH2_Q6IBW4-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2644509

Product Specific Information

Immunogen sequence: EAENEFLSLD DFPDSRTNVD LKNDQTPSEV LIIPLLPMAL VAPDEMEKNN NPLYSRQGEV LASRKDFRMN TCVPHPRGAF MLEPEGMS

Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 82%.

Target Information

Structural maintenance of chromosomes (SMC) and non-SMC condensin proteins associate into complexes that have been implicated in the process of chromosome condensation. A crucial prerequisite for accurate segregation of replicated sister chromatids is the condensation of the chromosomes into a manageable form prior to metaphase. The condensin I complex consists of two SMC subunits, SMC2 and SMC4, and three non-SMC subunits, CAP-H, CAP-G, and CAP-D2. An alternative complex, the condensin II complex, contains alternate non-SMC subunits, CAP-G2, CAP-H2, and CAP-D3. CAP-H2 is also known as Non-SMC condensin II complex, subunit H2 (NCAPH2) or kleisin beta isoform 2. The three non-SMC subunits in the condensing complexes form a regulatory subcomplex that is required to activate the SMC ATPases and to promote mitosis-specific chromatin binding of the holocomplex. The precise individual functions of each non-SMC protein in activation remain to be determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.