Thermo Fisher Scientific KDM5B Polyclonal Antibody
KDM5B 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western Blot, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합합니다. Rabbit IgG 기반으로 높은 특이성과 안정성을 제공하며, Protein A로 정제된 액상 형태입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human KDM5B/Jarid1B, amino acids 65–165 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
KDM5B is a histone demethylase that demethylates Lys-4 of histone H3, thereby playing a central role in the histone code. It does not demethylate histone H3 Lys-9 or Lys-27. KDM5B demethylates trimethylated, dimethylated, and monomethylated H3 Lys-4. It acts as a transcriptional corepressor for FOXG1B and PAX9. KDM5B promotes proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5, but may act as a tumor suppressor in melanoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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