Thermo Fisher Scientific Involucrin Monoclonal Antibody (SY5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 20 publications 20 publications

Immunohistochemistry (IHC)

-

View 23 publications 23 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 10 publications 10 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

2 µg/mL

-

Product Specifications

Species Reactivity

Dog, Human, Non-human primate, Pig, Rat

Published species

Human, Mouse, Rat, Sheep

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

SY5

Immunogen

Human keratinocytesinvolucrin if (typeof window.$mangular ===undefined|| !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson =[{targetFamily:Involucrin,uniProtId:P07476-1,ncbiNodeId:9606,antigenRange:1-585,antigenLength:585,antigenImageFileName:MA5-11803\_Involucrin\_P07476-1\_House\_mouse.svg,antigenImageFileNamePDP:MA5-11803\_Involucrin\_P07476-1\_House\_mouse\_PDP.jpeg,sortOrder:1}]; $mangular.isB2BCMGT = ``false``; $mangular.isEpitopesModalImageMultiSizeEnabled =true`;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982738

Product Specific Information

MA5-11803 targets Involucrin in ELISA, IF, IHC (P), IP, and WB applications and shows reactivity with Canine, Human, Non-human primate, Porcine, and Rat samples.

The MA5-11803 immunogen is human keratinocytes` involucrin.

Target Information

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.