Thermo Fisher Scientific CD15 Monoclonal Antibody (MMA (LeuM1))
인간 CD15 항원을 인식하는 Mouse IgM 단일클론 항체로, IHC, ICC/IF, Flow Cytometry 등에 사용 가능. 비결합형 액상 형태이며 0.1 mg/mL 농도로 제공. Hodgkin 병 진단 연구 등 다양한 세포 표지 연구에 활용.
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Thermo Fisher Scientific CD15 Monoclonal Antibody (MMA (LeuM1))
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | Assay-dependent | View publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MMA (LeuM1) |
| Immunogen | U937 histiocytic cell line |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10986179 |
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins, and proteoglycans of various cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and various tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X. Cells with high Le(x) surface expression show strong calcium-dependent self-aggregation, involved in embryo compaction and teratocarcinoma cell autoaggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands. CD15 expression is widely used to confirm Hodgkin’s disease diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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