Thermo Fisher Scientific TRIM28 Monoclonal Antibody (1D11)
TRIM28 단백질을 인식하는 Mouse monoclonal antibody(클론 1D11). Western blot, IHC, ELISA에 적합하며 Human, Mouse, Rat 시료 반응. Affinity chromatography로 정제된 액상형 항체로, PBS buffer에 보존 및 -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| ELISA | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D11 |
| Immunogen | TRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein sequence:
IVDPVEPHGE MKFQWDLNAW TKSAEAFGKI VAERPGTNST GPAPMAPPRA PGPLSKQGSG
SSQPMEVQEG YGFGSGDDPY SSAEPHVSGV KRSRSGEGEV SGLMRKVPRV SLERLDLDLT
ADSQPPVFKV FPGSTTEDYN LIVIERTarget Information
The KRAB (Kruppel-associated box) domain (~45 amino acids) is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription via interactions with KAP-1 (KRAB-associated protein 1), an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger.
KAP-1 forms complexes with KRAB-domain transcription factors to enhance repression efficiency and interacts with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directs KRAZ1 to centromeric heterochromatin foci containing HP1α, regulating transcriptional repression. Mutant KAP-1 that binds KRAB but not HP1 leads to random KRAZ1 distribution and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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