Thermo Fisher Scientific p300 Polyclonal Antibody
p300 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP, ChIP-Seq 등 다양한 응용에 적합합니다. 고순도 액상 형태로 제공되며, 4°C에서 보관합니다. 인간 E1A 관련 단백질 p300의 950–1000 잔기 영역을 면역원으로 사용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
| ChIP-sequencing (ChIP-Seq) | 10 µL/IP |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 950 and 1000 of human E1A-associated protein p300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 also acts as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), contributing to the stimulation of hypoxia-induced genes such as VEGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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