Applied Biosystems™

TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 50 mL PCR)

The Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one자세히 알아보기

The Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan™ Sample-to-SNP™ Kit combines DNA extraction and TaqMan™ chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan™™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems™ TaqMan™ Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 μL/ reaction and 20,000 PCR reactions at 5 μL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan™ Sample-to-SNP™ Kit.

사양

검출 방법Primer-probe

PCR 방법qPCR

중합효소AmpliTaq DNA Polymerase

반응 속도Fast or Standard

기술SNP Genotyping (Real-Time PCR-Based)

용도(장비)7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Fast Mode, StepOne™, Standard Mode, Veriti Thermal Cycler, StepOne™ Standard Mode, StepOne™ Fast Mode

유전형 분석 타겟SNPs (Unknown or Numerous), SNPs (Known)

패시브 참조 염료ROX (Pre-mixed)

제품라인Sample-to-SNP™, TaqMan™

제품 유형PCR Kit

수량1 kit

핫 스타트Built-In Hot Start

반응 수4000 Reactions (5 μL, PCR), 4000 Reactions (50 μL/Reaction, Sample Preparation)

샘플 종류Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood

부피20 mL (Sample Preparation), 50 mL (PCR)

Unit SizeEach