Thermo Fisher Scientific HP1 alpha Monoclonal Antibody (F.747.6)
HP1 alpha 단백질을 인식하는 Thermo Fisher Scientific의 F.747.6 단클론 항체입니다. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합하며, 인간 및 여러 종에 반응합니다. 고순도 친화 크로마토그래피 정제, 액상 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 1:25 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | F.747.6 |
| Immunogen | Synthetic peptide corresponding to the carboxy terminus of human HP1 alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 4.5 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10979640 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with HP1-gamma proteins.
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. Mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, which are heterochromatic adaptor molecules involved in gene silencing and chromatin structure.
High-affinity recognition of methylated histone H3 by HP1 requires a functional chromodomain, making it a specific interaction motif for the methyl epitope on lysine-9.
In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-mutant mouse fibroblasts but restored after reintroduction of catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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