Thermo Fisher Scientific CD163 Monoclonal Antibody (OTI1B4), TrueMAB
CD163 단백질을 표적으로 하는 Thermo Fisher Scientific의 TrueMAB 단일클론 항체. 인간 단핵구 및 대식세포에서 발현되는 CD163 검출에 적합. WB, IHC, ICC 등 다양한 응용 가능. 고순도 액상 형태로 안정적이며 연구용으로 최적화됨.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Miscellaneous (PubMed) | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B4 |
| Immunogen | Full length human recombinant protein of human CD163 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex.
CD163 protects tissues from free hemoglobin-mediated oxidative damage and may play a role in the uptake and recycling of iron via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme.
It is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells, and Langerhans cells.
CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes.
CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6, and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant.
CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, exhibiting higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype.
Furthermore, CD163 induces a cascade of intracellular signals involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL6 and CSF1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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