Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), PE, eBioscience

Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), PE, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496 / 563 / 615 / 661 / 737
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue, Red, Yellow series
• PE-Cyanine5 / 5.5 / 7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 780
• Request custom conjugation

Product Specific Information

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain is essential for assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates intracellular signaling leading to activation, proliferation, or apoptosis depending on conditions. Commonly used as a phenotypic marker for mouse T cells.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse thymocytes and splenocytes; ≤0.5 µg per test (100 µL final volume). Optimal titration recommended.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD3 complex is crucial for transducing antigen-recognition signals into T cells and regulating TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are structurally related members of the immunoglobulin superfamily encoded on chromosome 11. They contain cytoplasmic tails with tyrosine-based motifs mediating signal transduction. CD3 is expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Defects in CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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