Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), PE, eBioscience
Mouse CD3e 인식 단일클론 항체로 T 세포 표지 및 활성화 연구에 적합. PE 형광체 결합으로 Flow Cytometry 분석에 최적화. 고순도 친화 크로마토그래피 정제 제품이며 4°C 암소 보관. 다양한 형광체 포맷 제공.
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Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), PE, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 208 publications |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 145-2C11 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465495 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 496 / 563 / 615 / 661 / 737
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue, Red, Yellow series
- PE-Cyanine5 / 5.5 / 7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 780
- Request custom conjugation
Product Specific Information
The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain is essential for assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates intracellular signaling leading to activation, proliferation, or apoptosis depending on conditions. Commonly used as a phenotypic marker for mouse T cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse thymocytes and splenocytes; ≤0.5 µg per test (100 µL final volume). Optimal titration recommended.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD3 complex is crucial for transducing antigen-recognition signals into T cells and regulating TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are structurally related members of the immunoglobulin superfamily encoded on chromosome 11. They contain cytoplasmic tails with tyrosine-based motifs mediating signal transduction. CD3 is expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Defects in CD3 gene are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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