Thermo Fisher Scientific CD86 Monoclonal Antibody (BU63), PE-Alexa Fluor 700

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

BU63

Immunogen

B-lymphoblastoid cell line ARH 77

Conjugate

PE-Alexa Fluor™ 700 PE-Alexa Fluor™ 700 PE-Alexa Fluor™ 700

View additional formats

• APC
• FITC
• PE
• Request custom conjugation

Excitation/Emission Max

566/721 nm View spectra

Form

Liquid

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

RRID

AB_10392704

Target Information

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

Analyte Specific Reagent