Thermo Fisher Scientific rpL7a/SURF3 Polyclonal Antibody
Western blot, IHC, IP 등 다양한 응용에 적합한 Rabbit Polyclonal 항체. Human 및 Mouse 반응성. 항원 친화 크로마토그래피로 정제된 액상 제품으로, 4°C 보관 및 Wet ice 배송. IHC에서 Tris-EDTA(pH 9.0) 항원 회복 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 215 and the C-terminus (residue 266) of Human Ribosomal Protein L7a |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with Tris-EDTA (pH 9.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat, Chicken, and Bovine
Target Information
Cytoplasmic ribosomes, which catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit composed of four RNA species and approximately 80 distinct proteins. The encoded ribosomal protein is a component of the 60S subunit and belongs to the L7AE family. It can interact with nuclear hormone receptors, such as the thyroid hormone receptor, inhibiting their DNA-binding activity.
This gene is part of the surfeit gene cluster and co-transcribed with multiple small nucleolar RNA genes located within its introns. It can rearrange with the trk proto-oncogene to form the chimeric oncogene trk-2h, encoding an oncoprotein that fuses the N-terminus of ribosomal protein L7a with the receptor tyrosine kinase domain of trk. Multiple processed pseudogenes of this gene are dispersed throughout the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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