Thermo Fisher Scientific Phospho-Smad1 (Ser463) Polyclonal Antibody
Smad1 단백질의 Ser463 인산화 부위를 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Thermo Fisher Scientific Phospho-Smad1 (Ser463) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of Ser463 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by this gene belongs to the SMAD family, which includes proteins similar to the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma.
SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
This protein mediates the signals of bone morphogenetic proteins (BMPs), which are involved in cell growth, apoptosis, morphogenesis, development, and immune responses.
Upon BMP ligand binding, this protein is phosphorylated and activated by BMP receptor kinase, forming a complex with SMAD4 for transcriptional regulation.
It is targeted by SMAD-specific E3 ubiquitin ligases (SMURF1, SMURF2) and undergoes ubiquitination and proteasome-mediated degradation.
Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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