Thermo Fisher Scientific 4E-T/eIF4E-T Polyclonal Antibody
4E-T/eIF4E-T 단백질을 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, IP 등 다양한 응용에 사용 가능. 인산화되지 않은 상태의 단백질 검출에 적합하며, 1 mg/mL 농도의 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 925 and the C-terminus (residue 985) of human Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. It interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. The protein is predominantly cytoplasmic, with nuclear import regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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