Thermo Fisher Scientific HDAC2 Polyclonal Antibody
HDAC2 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, IP, ChIP-Seq 등 다양한 응용에 사용 가능. 4°C에서 보관하며, 액상 형태로 제공. 인간 HDAC2의 C-말단 영역(425-488 aa)을 면역원으로 제작됨.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
| ChIP-sequencing (ChIP-Seq) | 10 µL/IP |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 425 and the C-terminus (residue 488) of human Histone Deacetylase 2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
HDAC2 belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3, and H4).
This protein forms transcriptional repressor complexes by associating with various proteins, including YY1, a mammalian zinc-finger transcription factor.
It plays an important role in transcriptional regulation, cell cycle progression, and developmental events.
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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