Thermo Fisher Scientific HRI Recombinant Rabbit Monoclonal Antibody (7H3L3)
HRI 단백질을 인식하는 재조합 토끼 단클론 항체. Western blot에서 검증됨. 높은 특이성과 일관된 로트 간 재현성 제공. PBS 버퍼에 0.09% sodium azide 포함, 단기 4°C, 장기 -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 1–2 µg/mL
- View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Predicted Reactivity | Monkey, Horse, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7H3L3 |
| Immunogen | Protein corresponding to human eIF2AK1 / Heme regulated inhibitor (HRI) (aa271–630) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2664566 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
EIF2AK1 (HRI) is one of four kinases that phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to environmental stresses, leading to decreased protein synthesis.
HRI signaling is initiated by low heme levels and inhibited by the presence of heme. The HRI gene is localized to chromosome 7p22, where its 3′ end slightly overlaps the 3′ end of the JTV1 gene. The two genes are transcribed from opposite strands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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